bovine milk sevs  (PureTech Health PLC)

Journal: Membranes

Article Title: Identification of Suitable Internal Control miRNAs in Bovine Milk Small Extracellular Vesicles for Normalization in Quantitative Real-Time Polymerase Chain Reaction

doi: 10.3390/membranes13020185

Figure Lengend Snippet: Characterization of bovine milk small extracellular vesicles. Morphology of bovine milk sEVs by transmission electron microscopy analysis ( a ) and detection marker proteins in bovine milk sEVs by Western blot analysis. ( b ) TEM detected bilayer spherical shape sEVs in milk (scale bar shows 200 nm). WB analysis successfully detected surface-marker-protein CD63 and internal-marker-protein HSP70 by using anti-CD63 and –HSP70 antibodies. WB analysis also detected less contaminant protein apoA1 in bovine milk sEVs. TEM, transmission electron microscopy; WB, Western blot.

Article Snippet: Then, the bovine milk sEVs’ morphology was observed under an electron microscope, JEM-2100F (JEOL, Tokyo, Japan).

Techniques: Transmission Assay, Electron Microscopy, Marker, Western Blot

Journal: Extracellular vesicles and circulating nucleic acids

Article Title: Milk-borne small extracellular vesicles: kinetics and mechanisms of transport, distribution, and elimination

doi: 10.20517/evcna.2023.25

Figure Lengend Snippet: Tissue distribution of milk sEVs in mice. (A) Distribution of bovine milk sEVs loaded with fluorophore (ATTO)-labeled miR-375 in Balb/c mice. Modified from Manca et al . with permission from the original publisher, Nature Springer . To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ ; (B) Accumulation of enhanced green fluorescence protein (eGFP)-positive milk sEVs in peripheral tissues and the small intestinal mucosa in wild-type (WT) pups fostered to exosome and cargo tracking (ECT) dams and nursed for 17 days. ECT mice secrete sEVs labeled with an eGFP fusion protein in milk. WT pups fostered to WT dams served as controls. From Zhou et al . with permission from the original publisher, Frontiers Media SA . To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ .

Article Snippet: The pharmaceutical industry has recognized the potential for using bovine milk sEVs to deliver anti-cancer drugs, as evidenced by a $1 billion licensing agreement between Roche, Inc. and PureTech Health, Inc. [ ] .

Techniques: Labeling, Modification, Fluorescence

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: Characterization of milk sEVs. ( A ) TEM detected bilayer spherical shape sEVs in milk (Scale bar shows 0.2 µm). ( B ) WB analysis successfully detected milk sEV-surface-marker protein CD63 and internal protein HSP70 using antibodies, anti-CD63 (SC-31214, Santa Cruz Biotechnology), and anti–HSP70 (N27F3-4, Enzo Life Science), respectively. ( C ) The size distribution of milk sEVs as determined by NTA. A representative data from cow 2 is shown (mean peak size < 0.2 µm in diameter). TEM, transmission electron microscopy; WB, western blot; NTA, nanoparticle tracking analysis.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques: Marker, Transmission Assay, Electron Microscopy, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: Proteomic analysis of milk sEVs. The Venn diagram displays the number of identified proteins in milk sEVs.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: Comparative Vann diagram. Comparison of milk sEVs proteins in the current study with bovine milk sEVs dataset.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques: Comparison

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: The comparative cellular component of identified proteins in milk sEVs.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: Top 50 newly identified proteins in milk sEVs from late-stage lactating cows.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques: Ubiquitin Proteomics, Binding Assay, Conjugation Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: GO analyses. Biological process ( A ) and molecular function ( B ) of newly identified proteins in milk sEVs.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows

doi: 10.3390/ani11092506

Figure Lengend Snippet: Biological pathway analysis (Top 10) of newly identified proteins in milk sEVs.

Article Snippet: Raw data of proteomic analysis of bovine milk sEVs from cows 1, 2, and 3 were deposited in the Mendeley data repository (Mendeley Data, V1, doi: 10.17632/ 7c2 ddgwcgt.1) (Direct URL: https://data.mendeley.com/datasets/7c2ddgwcgt/1 , accessed on 7 October 2020).

Techniques: